Direct observation of proton pumping by a eukaryotic P-type ATPase
نویسندگان
چکیده
منابع مشابه
Proton Pumping P Systems
We propose here a (biologically inspired) model of P system called proton pumping P system that is a special case of evolution– communication P system. In cell biology there are transport mechanisms, involving protons. We generalize this idea by considering a few different types of protons. A proton pumping P system is, essentially, an evolution–communication P system where a special subset of ...
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The distribution of vacuolar H+ATPase in rat kidney was examined by immunocytochemistry using affinity-purified antibodies against the 31-, 56-, and 70-kD subunits of the bovine kidney proton pump. Proximal convoluted tubules were labeled over apical plasma membrane invaginations, and in the initial part of the thin descending limb, apical and basolateral plasma membranes were moderately staine...
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F-ATPases synthesize ATP from ADP and phosphate coupled with an electrochemical proton gradient in bacterial or mitochondrial membranes and can hydrolyse ATP to form the gradient. F-ATPases consist of a catalytic F1 and proton channel F0 formed from the alpha3beta3gammadelta and ab2c10 subunit complexes, respectively. The rotation of gammaepsilonc10 couples catalyses and proton transport. Consi...
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Vacuolar-type ATPases V1V0 (V-ATPases) are found ubiquitously in the endomembrane organelles of eukaryotic cells. In this study, we genetically introduced a His tag and a biotin tag onto the c and G subunits, respectively, of Saccharomyces cerevisiae V-ATPase. Using this engineered enzyme, we observed directly the continuous counter-clockwise rotation of an actin filament attached to the G subu...
متن کاملPma1, a P-type proton ATPase, is a determinant of chronological life span in fission yeast.
Chronological life span is defined by how long a cell can survive in a non-dividing state. In yeast, it is measured by viability after entry into stationary phase. To date, some factors affecting chronological life span have been identified; however, the molecular details of how these factors regulate chronological life span have not yet been elucidated clearly. Because life span is a complicat...
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ژورنال
عنوان ژورنال: Science
سال: 2016
ISSN: 0036-8075,1095-9203
DOI: 10.1126/science.aad6429